Addgene - pMXs-GLIS1 Plasmid Data

Plasmid 30166: pMXs-GLIS1

Gene/insert name:
GLIS1
Alt name:
FLJ36155
Insert size:
1931
Species:
H. sapiens (human)
GenBank ID:
NM_147193
Entrez Gene:
GLIS1 (FLJ36155)
Vector backbone:
pMXs-gw
(Search Vector Database)
Backbone manufacturer:
Shinya Yamanaka
Vector type:
Retroviral
Backbone size w/o insert (bp):
4700
Cloning method:
Gateway Cloning
Cloning site 5':
att B1
Site destroyed during cloning:
Unknown
Cloning site 3':
att B2
Site destroyed during cloning:
Unknown
5' sequencing primer:
pMX-S1811 List of Sequencing Primers
3' sequencing primer:
pMX-AS3200
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Gateway cassette is from Invitrogen Corporation.
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Shinya Yamanaka
Terms and Licenses:
MTA

Comments:

pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Gateway casette is from Invitrogen Corporation.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1. Maekawa et al (Nature. 2011 . 474(7350):225-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 30166" in your Materials and Methods section.