Addgene - pET-3c-rhVTN-N Plasmid Data

Plasmid 30225: pET-3c-rhVTN-N

Gene/insert name:
rhVitronectin with N terminus cleavage
Alt name:
VTN
Alt name:
rhVTN-N
Alt name:
VTN-N
Insert size:
1254
Species:
H. sapiens (human)
Entrez Gene:
VTN (V75, VN, VNT)
Mutation:
deleted amino acids: 20*-62
Vector backbone:
pET-3c
(Search Vector Database)
Backbone manufacturer:
Novagen
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
4607
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
NdeI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
DH5a in LB media
High or low copy:
Low Copy
Sequence: View sequences (2)
Principal Investigator:
James Thomson
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Comments:

Note that the first 19 amino acids are the signal peptide for VTN, which is cleaved off on the mature protein during normally protein synthesis in mammalian cells. That means wild type mature VTN starts from aa20. That is why none of the four constructs have amino acid 1-19 and why pET-3c-rhVTN-N is a deletion of aa 20-62 from WT VTN.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Chemically defined conditions for human iPSC derivation and culture. Chen et al (Nat Methods. 2011 Apr 10. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 30225" in your Materials and Methods section.