pEGFP-C1-PRKAA1ΔC
(Plasmid #30306)

• Depositing Lab: Jay Brenman
• Publication: Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-42.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AMP-activated Protein Kinase α1 ΔC
• Alt name: AMPKα1ΔC
• Alt name: AMPKα1
• Alt name: AMPK
• Species: R. norvegicus (rat)
• Insert Size (bp): 1600
• Mutation: deleted amino acids Pro 526 to Gln 548
• GenBank ID: NP_001013385
• Entrez Gene: Prkaa1 (a.k.a. AMPKalpha1)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry