Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
Holiday Schedule: Addgene will be closed November 28th & 29th for the Thanksgiving holiday. Order processing and shipping may be delayed the week of November 25th - 29th. If you have any questions, please contact us at [email protected].

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pLKO.1 GFP shRNA Citations (39)

Plasmid Article: The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1.
Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled L, Sabatini DM Science. 2008 Jun 13. 320(5882):1496-501.
PubMed Journal

Articles Citing pLKO.1 GFP shRNA

Articles
p53-mediated heterochromatin reorganization regulates its cell fate decisions. Mungamuri SK, Benson EK, Wang S, Gu W, Lee SW, Aaronson SA. Nat Struct Mol Biol. 2012 Apr 1;19(5):478-84, S1. doi: 10.1038/nsmb.2271. PubMed
MicroRNA-34b inhibits pancreatic cancer metastasis through repressing Smad3. Liu C, Cheng H, Shi S, Cui X, Yang J, Chen L, Cen P, Cai X, Lu Y, Wu C, Yao W, Qin Y, Liu L, Long J, Xu J, Li M, Yu X. Curr Mol Med. 2013 May;13(4):467-78. PubMed
Proteome identification of proteins interacting with histone methyltransferase SET8. Qin Y, Ouyang H, Liu J, Xie Y. Acta Biochim Biophys Sin (Shanghai). 2013 Apr;45(4):303-8. doi: 10.1093/abbs/gmt011. Epub 2013 Feb 17. PubMed
Rapamycin-treated human endothelial cells preferentially activate allogeneic regulatory T cells. Wang C, Yi T, Qin L, Maldonado RA, von Andrian UH, Kulkarni S, Tellides G, Pober JS. J Clin Invest. 2013 Apr;123(4):1677-93. doi: 10.1172/JCI66204. Epub 2013 Mar 8. PubMed
Calretinin is essential for mesothelioma cell growth/survival in vitro: a potential new target for malignant mesothelioma therapy? Blum W, Schwaller B. Int J Cancer. 2013 Nov;133(9):2077-88. doi: 10.1002/ijc.28218. Epub 2013 Jul 9. PubMed
Rapamycin antagonizes TNF induction of VCAM-1 on endothelial cells by inhibiting mTORC2. Wang C, Qin L, Manes TD, Kirkiles-Smith NC, Tellides G, Pober JS. J Exp Med. 2014 Mar 10;211(3):395-404. doi: 10.1084/jem.20131125. Epub 2014 Feb 10. PubMed
Quantitative proteomics identifies NCOA4 as the cargo receptor mediating ferritinophagy. Mancias JD, Wang X, Gygi SP, Harper JW, Kimmelman AC. Nature. 2014 May 1;509(7498):105-9. doi: 10.1038/nature13148. Epub 2014 Mar 30. PubMed
MicroRNA-26a promotes anoikis in human hepatocellular carcinoma cells by targeting alpha5 integrin. Zhang X, Cheng SL, Bian K, Wang L, Zhang X, Yan B, Jia LT, Zhao J, Gammoh N, Yang AG, Zhang R. Oncotarget. 2015 Feb 10;6(4):2277-89. PubMed
Polarity Protein Scrib Facilitates Endothelial Inflammatory Signaling. Kruse C, Kurz AR, Palfi K, Humbert PO, Sperandio M, Brandes RP, Fork C, Michaelis UR. Arterioscler Thromb Vasc Biol. 2015 Sep;35(9):1954-62. doi: 10.1161/ATVBAHA.115.305678. Epub 2015 Jul 23. PubMed
The Deacetylase Sirtuin 1 Regulates Human Papillomavirus Replication by Modulating Histone Acetylation and Recruitment of DNA Damage Factors NBS1 and Rad51 to Viral Genomes. Langsfeld ES, Bodily JM, Laimins LA. PLoS Pathog. 2015 Sep 25;11(9):e1005181. doi: 10.1371/journal.ppat.1005181. eCollection 2015 Sep. PubMed
Ferritinophagy via NCOA4 is required for erythropoiesis and is regulated by iron dependent HERC2-mediated proteolysis. Mancias JD, Pontano Vaites L, Nissim S, Biancur DE, Kim AJ, Wang X, Liu Y, Goessling W, Kimmelman AC, Harper JW. Elife. 2015 Oct 5;4. doi: 10.7554/eLife.10308. PubMed
Integrated genomics approach to identify biologically relevant alterations in fewer samples. Chandrani P, Upadhyay P, Iyer P, Tanna M, Shetty M, Raghuram GV, Oak N, Singh A, Chaubal R, Ramteke M, Gupta S, Dutt A. BMC Genomics. 2015 Nov 14;16(1):936. doi: 10.1186/s12864-015-2138-4. PubMed
NIPBL Controls RNA Biogenesis to Prevent Activation of the Stress Kinase PKR. Yuen KC, Xu B, Krantz ID, Gerton JL. Cell Rep. 2016 Jan 5;14(1):93-102. doi: 10.1016/j.celrep.2015.12.012. Epub 2015 Dec 24. PubMed
The Endoplasmic Reticulum Chaperone Calnexin Is a NADPH Oxidase NOX4 Interacting Protein. Prior KK, Wittig I, Leisegang MS, Groenendyk J, Weissmann N, Michalak M, Jansen-Durr P, Shah AM, Brandes RP. J Biol Chem. 2016 Mar 25;291(13):7045-59. doi: 10.1074/jbc.M115.710772. Epub 2016 Feb 9. PubMed
Biophysical changes reduce energetic demand in growth factor-deprived lymphocytes. Hecht VC, Sullivan LB, Kimmerling RJ, Kim DH, Hosios AM, Stockslager MA, Stevens MM, Kang JH, Wirtz D, Vander Heiden MG, Manalis SR. J Cell Biol. 2016 Feb 15;212(4):439-47. doi: 10.1083/jcb.201506118. PubMed
Oncogenic function of the homeobox A13-long noncoding RNA HOTTIP-insulin growth factor-binding protein 3 axis in human gastric cancer. Wang SS, Wuputra K, Liu CJ, Lin YC, Chen YT, Chai CY, Lin CS, Kuo KK, Tsai MH, Wang SW, Chen KK, Miyoshi H, Nakamura Y, Saito S, Hanafusa T, Wu DC, Lin CS, Yokoyama KK. Oncotarget. 2016 Jun 14;7(24):36049-36064. doi: 10.18632/oncotarget.9102. PubMed
EZH2-mediated Puma gene repression regulates non-small cell lung cancer cell proliferation and cisplatin-induced apoptosis. Liu H, Li W, Yu X, Gao F, Duan Z, Ma X, Tan S, Yuan Y, Liu L, Wang J, Zhou X, Yang Y. Oncotarget. 2016 Aug 30;7(35):56338-56354. doi: 10.18632/oncotarget.10841. PubMed
Pancreatic stellate cells support tumour metabolism through autophagic alanine secretion. Sousa CM, Biancur DE, Wang X, Halbrook CJ, Sherman MH, Zhang L, Kremer D, Hwang RF, Witkiewicz AK, Ying H, Asara JM, Evans RM, Cantley LC, Lyssiotis CA, Kimmelman AC. Nature. 2016 Aug 25;536(7617):479-83. PubMed
AEG-1 promotes mesenchymal transition through the activation of Rho GTPases in human glioblastoma cells. Park SY, Choi M, Park D, Jeong M, Ahn KS, Lee J, Fisher PB, Yun M, Lee SG. Oncol Rep. 2016 Nov;36(5):2641-2646. doi: 10.3892/or.2016.5106. Epub 2016 Sep 19. PubMed
Snail1-dependent p53 repression regulates expansion and activity of tumour-initiating cells in breast cancer. Ni T, Li XY, Lu N, An T, Liu ZP, Fu R, Lv WC, Zhang YW, Xu XJ, Grant Rowe R, Lin YS, Scherer A, Feinberg T, Zheng XQ, Chen BA, Liu XS, Guo QL, Wu ZQ, Weiss SJ. Nat Cell Biol. 2016 Nov;18(11):1221-1232. doi: 10.1038/ncb3425. Epub 2016 Oct 17. PubMed
Targeting MCL-1 sensitizes human esophageal squamous cell carcinoma cells to cisplatin-induced apoptosis. Yu X, Li W, Xia Z, Xie L, Ma X, Liang Q, Liu L, Wang J, Zhou X, Yang Y, Liu H. BMC Cancer. 2017 Jun 28;17(1):449. doi: 10.1186/s12885-017-3442-y. PubMed
Condensin II is anchored by TFIIIC and H3K4me3 in the mammalian genome and supports the expression of active dense gene clusters. Yuen KC, Slaughter BD, Gerton JL. Sci Adv. 2017 Jun 21;3(6):e1700191. doi: 10.1126/sciadv.1700191. eCollection 2017 Jun. PubMed
Reprogramming Antagonizes the Oncogenicity of HOXA13-Long Noncoding RNA HOTTIP Axis in Gastric Cancer Cells. Wu DC, Wang SSW, Liu CJ, Wuputra K, Kato K, Lee YL, Lin YC, Tsai MH, Ku CC, Lin WH, Wang SW, Kishikawa S, Noguchi M, Wu CC, Chen YT, Chai CY, Lin CS, Kuo KK, Yang YH, Miyoshi H, Nakamura Y, Saito S, Nagata K, Lin CS, Yokoyama KK. Stem Cells. 2017 Oct;35(10):2115-2128. doi: 10.1002/stem.2674. Epub 2017 Aug 23. PubMed
MicroRNA miR-128 represses LINE-1 (L1) retrotransposition by down-regulating the nuclear import factor TNPO1. Idica A, Sevrioukov EA, Zisoulis DG, Hamdorf M, Daugaard I, Kadandale P, Pedersen IM. J Biol Chem. 2017 Dec 15;292(50):20494-20508. doi: 10.1074/jbc.M117.807677. Epub 2017 Oct 3. PubMed
Deguelin, an Aurora B Kinase Inhibitor, Exhibits Potent Anti-Tumor Effect in Human Esophageal Squamous Cell Carcinoma. Yu X, Liang Q, Liu W, Zhou L, Li W, Liu H. EBioMedicine. 2017 Dec;26:100-111. doi: 10.1016/j.ebiom.2017.10.030. Epub 2017 Nov 3. PubMed
Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer. Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA. Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 10.1016/j.devcel.2017.10.027. PubMed
HSF1 Is Essential for Myeloma Cell Survival and A Promising Therapeutic Target. Fok JHL, Hedayat S, Zhang L, Aronson LI, Mirabella F, Pawlyn C, Bright MD, Wardell CP, Keats JJ, De Billy E, Rye CS, Chessum NEA, Jones K, Morgan GJ, Eccles SA, Workman P, Davies FE. Clin Cancer Res. 2018 May 15;24(10):2395-2407. doi: 10.1158/1078-0432.CCR-17-1594. Epub 2018 Feb 1. PubMed
Deguelin suppresses angiogenesis in human hepatocellular carcinoma by targeting HGF-c-Met pathway. Li M, Yu X, Li W, Liu T, Deng G, Liu W, Liu H, Gao F. Oncotarget. 2017 Oct 26;9(1):152-166. doi: 10.18632/oncotarget.22077. eCollection 2018 Jan 2. PubMed
Capsaicin inhibits glycolysis in esophageal squamous cell carcinoma by regulating hexokinase2 expression. Mao X, Zhu H, Luo D, Ye L, Yin H, Zhang J, Zhang Y, Zhang Y. Mol Med Rep. 2018 Apr;17(4):6116-6121. doi: 10.3892/mmr.2018.8574. Epub 2018 Feb 8. PubMed
De novo mutations in MED13, a component of the Mediator complex, are associated with a novel neurodevelopmental disorder. Snijders Blok L, Hiatt SM, Bowling KM, Prokop JW, Engel KL, Cochran JN, Bebin EM, Bijlsma EK, Ruivenkamp CAL, Terhal P, Simon MEH, Smith R, Hurst JA, McLaughlin H, Person R, Crunk A, Wangler MF, Streff H, Symonds JD, Zuberi SM, Elliott KS, Sanders VR, Masunga A, Hopkin RJ, Dubbs HA, Ortiz-Gonzalez XR, Pfundt R, Brunner HG, Fisher SE, Kleefstra T, Cooper GM. Hum Genet. 2018 May;137(5):375-388. doi: 10.1007/s00439-018-1887-y. Epub 2018 May 8. PubMed
Aspartate is an endogenous metabolic limitation for tumour growth. Sullivan LB, Luengo A, Danai LV, Bush LN, Diehl FF, Hosios AM, Lau AN, Elmiligy S, Malstrom S, Lewis CA, Vander Heiden MG. Nat Cell Biol. 2018 Jul;20(7):782-788. doi: 10.1038/s41556-018-0125-0. Epub 2018 Jun 25. PubMed

Associated Plasmids

Deguelin attenuates non-small cell lung cancer cell metastasis through inhibiting the CtsZ/FAK signaling pathway. Li W, Yu X, Ma X, Xie L, Xia Z, Liu L, Yu X, Wang J, Zhou H, Zhou X, Yang Y, Liu H. Cell Signal. 2018 Oct;50:131-141. doi: 10.1016/j.cellsig.2018.07.001. Epub 2018 Jul 7. PubMed
Repression of Noxa by Bmi1 contributes to deguelin-induced apoptosis in non-small cell lung cancer cells. Li W, Yu X, Xia Z, Yu X, Xie L, Ma X, Zhou H, Liu L, Wang J, Yang Y, Liu H. J Cell Mol Med. 2018 Dec;22(12):6213-6227. doi: 10.1111/jcmm.13908. Epub 2018 Sep 25. PubMed
Oncogenic KRAS supports pancreatic cancer through regulation of nucleotide synthesis. Santana-Codina N, Roeth AA, Zhang Y, Yang A, Mashadova O, Asara JM, Wang X, Bronson RT, Lyssiotis CA, Ying H, Kimmelman AC. Nat Commun. 2018 Nov 23;9(1):4945. doi: 10.1038/s41467-018-07472-8. PubMed
Calretinin Functions in Malignant Mesothelioma Cells Cannot Be Replaced by the Closely Related Ca(2+)-Binding Proteins Calbindin-D28k and Parvalbumin. Worthmuller J, Oberson A, Salicio V, Blum W, Schwaller B. Int J Mol Sci. 2018 Dec 12;19(12). pii: ijms19124015. doi: 10.3390/ijms19124015. PubMed
Calretinin promotes invasiveness and EMT in malignant mesothelioma cells involving the activation of the FAK signaling pathway. Worthmuller J, Blum W, Pecze L, Salicio V, Schwaller B. Oncotarget. 2018 Nov 20;9(91):36256-36272. doi: 10.18632/oncotarget.26332. eCollection 2018 Nov 20. PubMed
NCOA4 maintains murine erythropoiesis via cell autonomous and non-autonomous mechanisms. Santana-Codina N, Gableske S, Quiles Del Rey M, Malachowska B, Jedrychowski MP, Biancur DE, Schmidt PJ, Fleming MD, Fendler W, Harper JW, Kimmelman AC, Mancias JD. Haematologica. 2019 Jan 10. pii: haematol.2018.204123. doi: 10.3324/haematol.2018.204123. PubMed
miR-128 Restriction of LINE-1 (L1) Retrotransposition Is Dependent on Targeting hnRNPA1 mRNA. Fung L, Guzman H, Sevrioukov E, Idica A, Park E, Bochnakian A, Daugaard I, Jury D, Mortazavi A, Zisoulis DG, Pedersen IM. Int J Mol Sci. 2019 Apr 21;20(8). pii: ijms20081955. doi: 10.3390/ijms20081955. PubMed
TDP-43 is Required for Mammary Gland Repopulation and Proliferation of Mammary Epithelial Cells. Zhao L, Li L, Xu H, Ke H, Zou L, Yang Q, Shen CJ, Nie J, Jiao B. Stem Cells Dev. 2019 Jul 15;28(14):944-953. doi: 10.1089/scd.2019.0011. Epub 2019 Jun 11. PubMed

If you have published an article using this plasmid, please email us at [email protected] to have your article added to this page.