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(-6kb/-58)-GFP
(Plasmid #30460)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 30460 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HNF1a promoter
  • Alt name
    LFB1 promoter
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    5896
  • GenBank ID
    AY471617

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The GFP variant in the plasmid backbone, pCSGFP2, contains the F64L and the S65T mutations resulting in 35 times brighter green emission (as described in Ikawa M et al., Curr. Top. Dev. Biol. 44 (1999), pp. 1–20)

This construct contains the 5′ EcoRI fragment of the genomic clone XLF 4b (as described in Zapp D et al., Gene 134 (1993), pp. 251–256) linked to the EcoRI–HindIII fragment of the promoter clone 5Δ594 (as described in Zapp D et al., Mol. Cell. Biol. 13 (1993), pp. 6416–6426). By this strategy the sequence from −6 kbp to −58 bp upstream of the translation initiation codon of the HNF1α gene is linked to the GFP reporter present in pCSGFP2.

Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    (-6kb/-58)-GFP was a gift from Gerhart Ryffel (Addgene plasmid # 30460 ; http://n2t.net/addgene:30460 ; RRID:Addgene_30460)
  • For your References section:

    Distinct promoter elements mediate endodermal and mesodermal expression of the HNF1alpha promoter in transgenic Xenopus. Ryffel GU, Lingott A. Mech Dev. 2000 Jan . 90(1):65-75. 10.1016/S0925-4773(99)00230-0 PubMed 10585563