(-6kb/-58)-GFP
(Plasmid #30460)

• Depositing Lab: Gerhart Ryffel
• Publication: Ryffel et al Mech Dev. 2000 Jan . 90(1):65-75.

Backbone

• Vector backbone: pCSGFP2
• Backbone size w/o insert (bp): 3812
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HNF1a promoter
• Alt name: LFB1 promoter
• Species: X. laevis (frog)
• Insert Size (bp): 5896
• GenBank ID: AY471617

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The GFP variant in the plasmid backbone, pCSGFP2, contains the F64L and the S65T mutations resulting in 35 times brighter green emission (as described in Ikawa M et al., Curr. Top. Dev. Biol. 44 (1999), pp. 1–20)

This construct contains the 5′ EcoRI fragment of the genomic clone XLF 4b (as described in Zapp D et al., Gene 134 (1993), pp. 251–256) linked to the EcoRI–HindIII fragment of the promoter clone 5Δ594 (as described in Zapp D et al., Mol. Cell. Biol. 13 (1993), pp. 6416–6426). By this strategy the sequence from −6 kbp to −58 bp upstream of the translation initiation codon of the HNF1α gene is linked to the GFP reporter present in pCSGFP2.

Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.