|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||30460||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3812
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHNF1a promoter
Alt nameLFB1 promoter
SpeciesX. laevis (frog)
Insert Size (bp)5896
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
The GFP variant in the plasmid backbone, pCSGFP2, contains the F64L and the S65T mutations resulting in 35 times brighter green emission (as described in Ikawa M et al., Curr. Top. Dev. Biol. 44 (1999), pp. 1–20)
This construct contains the 5′ EcoRI fragment of the genomic clone XLF 4b (as described in Zapp D et al., Gene 134 (1993), pp. 251–256) linked to the EcoRI–HindIII fragment of the promoter clone 5Δ594 (as described in Zapp D et al., Mol. Cell. Biol. 13 (1993), pp. 6416–6426). By this strategy the sequence from −6 kbp to −58 bp upstream of the translation initiation codon of the HNF1α gene is linked to the GFP reporter present in pCSGFP2.
Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:(-6kb/-58)-GFP was a gift from Gerhart Ryffel (Addgene plasmid # 30460 ; http://n2t.net/addgene:30460 ; RRID:Addgene_30460)
For your References section:Distinct promoter elements mediate endodermal and mesodermal expression of the HNF1alpha promoter in transgenic Xenopus. Ryffel GU, Lingott A. Mech Dev. 2000 Jan . 90(1):65-75. 10.1016/S0925-4773(99)00230-0 PubMed 10585563