Addgene - pcDNA-HDAC6.ΔBuz-FLAG Plasmid Data

Plasmid 30484: pcDNA-HDAC6.ΔBuz-FLAG

Gene/insert name:
HDAC6
Species:
H. sapiens (human)
Entrez Gene:
HDAC6 (JM21, HD6)
Fusion protein or tag:
FLAG
Terminal:
C terminal on insert
Mutation:
Deletion of the C-terminal Ubiquitin binding domain- aa1044-1273 (BUZ domain)
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Tso-Pang Yao
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The deacetylase HDAC6 regulates aggresome formation and cell viability in response to misfolded protein stress. Kawaguchi et al (Cell. 2003 Dec 12. 115(6):727-38. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 30484" in your Materials and Methods section.