Addgene - EGFP-Tubulin.K40R Plasmid Data

Plasmid 30488: EGFP-Tubulin.K40R

Gene/insert name:
α-tubulin
Alt name:
TUBA1B
Alt name:
K-ALPHA-1
Species:
H. sapiens (human)
Fusion protein or tag:
EGFP
Terminal:
N terminal on insert
Mutation:
K40R (Lys40Arg) Acetylation-resistant tubulin mutant
Vector backbone:
pcDNA3.1(+)
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5428
Cloning site 5':
NheI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
EGFP-C List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Tso-Pang Yao
Terms and Licenses:
MTA

Comments:

EGFP-tubulin was cloned out from Addgene plasmid #12298: pIRESneo-EGFP-α tubulin 1β (Patricia Wadsworth).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The microtubule-associated histone deacetylase 6 (HDAC6) regulates epidermal growth factor receptor (EGFR) endocytic trafficking and degradation. Gao et al (J Biol Chem. 2010 Apr 9. 285(15):11219-26. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 30488" in your Materials and Methods section.