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lacI-PT5-gusA-pBAV1k
(Plasmid #30501)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 30501 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBAV1k
  • Backbone size w/o insert (bp) 2792
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta-glucuronidase
  • Alt name
    gusA
  • Alt name
    pIM1440
  • Species
    Escherichia coli
  • Insert Size (bp)
    1796
  • GenBank ID
    JF828582
  • Entrez Gene
    gusA (a.k.a. ECO111_2086)
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI, EcoRI, NheI, SphI (not destroyed)
  • 3′ cloning site SpeI, PstI, ClaI, HindIII (not destroyed)
  • 5′ sequencing primer gacgaactccaattcactgttccttgc
  • 3′ sequencing primer ggagagcgttcaccgacaaacaacag
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This lab.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

In our experience, plasmid yields are highest when cells are harvested at late log stage.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lacI-PT5-gusA-pBAV1k was a gift from Ichiro Matsumura (Addgene plasmid # 30501 ; http://n2t.net/addgene:30501 ; RRID:Addgene_30501)
  • For your References section:

    Expression vectors for the engineering of genes and genomes in Acinetobacter baylyi ADP1. Murin CD, Segal K, Bryksin A, Matsumura I. Appl Environ Microbiol. 2011 Oct 21. 10.1128/AEM.05597-11 PubMed 22020504