Addgene - araC-pBAD-gusA-pBAV1k Plasmid Data

Plasmid 30504: araC-pBAD-gusA-pBAV1k

Gene/insert name:
beta-glucoronidase
Alt name:
gusA
Alt name:
pIM1442
Insert size:
1796
Species:
Escherichia coli
GenBank ID:
JF828585
Entrez Gene:
gusA (ECO111_2086)
Fusion protein or tag:
His
Terminal:
N terminal on insert
Vector backbone:
pBAV1k
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
2792
Cloning site 5':
EcoRI, XbaI
Site destroyed during cloning:
No
Cloning site 3':
SpeI, PstI
Site destroyed during cloning:
No
5' sequencing primer:
GACGAACTCCAATTCACTGTTCCTTGC List of Sequencing Primers
3' sequencing primer:
GGAGAGCGTTCACCGACAAACAACAG
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
Any strain that has a LacI repressor.
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
This lab.
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Ichiro Matsumura
Terms and Licenses:
MTA

Comments:

In our experience, plasmid yields are highest when cells are harvested at late log stage.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Expression vectors for the engineering of genes and genomes in Acinetobacter baylyi ADP1. Murin et al (Appl Environ Microbiol. 2011 Oct 21. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 30504" in your Materials and Methods section.