Addgene - pFUS_B7 Plasmid Data

Plasmid 31024: pFUS_B7

Gene/insert name:
LacZ + BsaI restriction sites
Insert size:
459
Vector backbone:
pCR8
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Backbone size w/o insert (bp):
2447
Cloning site 5':
AflII
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
pCR8_F1 (ttgatgcctggcagttccct) List of Sequencing Primers
3' sequencing primer:
pCR8_R1 (cgaaccgaacaggcttatgt)
Bacterial resistance(s)
Spectinomycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
DH10B/LB+antibiotics
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Adam Bogdanove
Principal Investigator:
Daniel Voytas
Terms and Licenses:
MTA
Cellectis Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak et al (Nucleic Acids Res. 2011 Apr 14. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31024" in your Materials and Methods section.