Addgene - pNK2 Plasmid Data

Plasmid 31037: pNK2

Gene/insert name:
repeat NK2
Insert size:
116
Species:
Xanthamonas oryzae
Vector backbone:
pTC14
(Search Vector Database)
Backbone size w/o insert (bp):
2989
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
pTC14_F2 (caagcctgattgggagaaaa) List of Sequencing Primers
Bacterial resistance(s)
Tetracycline
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
Top10/LB+antibiotics
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Adam Bogdanove
Principal Investigator:
Daniel Voytas
Terms and Licenses:
MTA
Cellectis Limited Use Label License

Comments:

IMPORTANT NOTE!: Restriction digests show that the pNK series of plasmids is actually about 2.5kb, not 3.1kb as the sequence-based map below suggests. All important features are present in the plasmid and the construct should function as reported despite this size discrepancy.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak et al (Nucleic Acids Res. 2011 Apr 14. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31037" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

27963: pLenti-EF1a-Backbone(NG)