|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31053||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4800
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHNF4A P2 promoter
SpeciesH. sapiens (human)
Insert Size (bp)285
Mutation-285 to -1 of P2 HNF4A promoter with mutation -192C>G
Entrez GeneHNF4A (a.k.a. FRTS4, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HNF4A_P2-285/-192 was a gift from Gerhart Ryffel (Addgene plasmid # 31053 ; http://n2t.net/addgene:31053 ; RRID:Addgene_31053)
For your References section:Novel monogenic diabetes mutations in the P2 promoter of the HNF4A gene are associated with impaired function in vitro. Wirsing A, Johnstone KA, Harries LW, Ellard S, Ryffel GU, Stanik J, Gasperikova D, Klimes I, Murphy R. Diabet Med. 2010 Jun . 27(6):631-5. 10.1111/j.1464-5491.2010.03003.x PubMed 20546279