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AnkyrinB-2XHA
(Plasmid #31057)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31057 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Modified pEGFP-N1 (see note)
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ankyrin B
  • Alt name
    ankyrin 2
  • Species
    H. sapiens (human)
  • Tag / Fusion Protein
    • 2XHA (C terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

HA-ankyrin-B was prepared based on an ankyrin-B-GFP template in a pEGFP-N1 vector (Mohler et al., 2002). GFP was cut out between the PmeI and the NotI sites and replaced with a 2XHA-tag encoding sequence (YPYDVPDYA) with a PmeI site on its 5′ end and a stop codon followed by a NotI site on the 3′ end that was synthesized by PCR.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AnkyrinB-2XHA was a gift from Vann Bennett (Addgene plasmid # 31057 ; http://n2t.net/addgene:31057 ; RRID:Addgene_31057)
  • For your References section:

    Ankyrin-B interactions with spectrin and dynactin-4 are required for dystrophin-based protection of skeletal muscle from exercise injury. Ayalon G, Hostettler JD, Hoffman J, Kizhatil K, Davis JQ, Bennett V. J Biol Chem. 2011 Mar 4. 286(9):7370-8. 10.1074/jbc.M110.187831 PubMed 21186323