AnkyrinG-190-GFP
(Plasmid #31059)

• Depositing Lab: Vann Bennett
• Publication: Mohler et al J Biol Chem. 2002 Mar 22. 277(12):10599-607.

Backbone

• Vector backbone: pEGFP-N2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ankyrin G
• Species: R. norvegicus (rat)
• Entrez Gene: Ank3 (a.k.a. ANK-3)
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: PmeI (unknown if destroyed)
• 5′ sequencing primer: CMV-Fwd
• 3′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry