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AnkyrinG-190-GFP
(Plasmid #31059)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pEGFP-N2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Ankyrin G
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Ank3
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site PmeI (unknown if destroyed)
  • 5′ sequencing primer CMV-Fwd
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AnkyrinG-190-GFP was a gift from Vann Bennett (Addgene plasmid # 31059)
  • For your References section:

    The ankyrin-B C-terminal domain determines activity of ankyrin-B/G chimeras in rescue of abnormal inositol 1,4,5-trisphosphate and ryanodine receptor distribution in ankyrin-B (-/-) neonatal cardiomyocytes. Mohler PJ, Gramolini AO, Bennett V. J Biol Chem. 2002 Mar 22. 277(12):10599-607. 10.1074/jbc.M110958200 PubMed 11781319