Addgene - AnkyrinG-190-GFP Plasmid Data

Plasmid 31059: AnkyrinG-190-GFP

Gene/insert name:
Ankyrin G
Species:
R. norvegicus (rat)
Entrez Gene:
Ank3 ()
Fusion protein or tag:
EGFP
Terminal:
C terminal on backbone
Vector backbone:
pEGFP-N2
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
EcoRI
Site destroyed during cloning:
Unknown
Cloning site 3':
PmeI
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV-Fwd List of Sequencing Primers
3' sequencing primer:
EGFP-N
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Vann Bennett
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The ankyrin-B C-terminal domain determines activity of ankyrin-B/G chimeras in rescue of abnormal inositol 1,4,5-trisphosphate and ryanodine receptor distribution in ankyrin-B (-/-) neonatal cardiomyocytes. Mohler et al (J Biol Chem. 2002 Mar 22. 277(12):10599-607. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31059" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

22907: pAAV-hSyn-RFP

31070: beta2-spectrin-HA

31057: AnkyrinB-2XHA

13030: pcDNA3-CFP

35097: ampkar