Addgene - pcDNA6.2-GW/EmGFP-miR-SUMO23 Plasmid Data

Plasmid 31073: pcDNA6.2-GW/EmGFP-miR-SUMO23

Gene/insert name:
SUMO2/3 microRNA
Alt name:
SUMO2/3
shRNA sequence:
SUMO2: GATCTGCCTCATTGACAAAC; SUMO3: AATCGAATCTGCCTCATTGAC
Species:
H. sapiens (human)
Entrez Gene:
SUMO2 (HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A)
SUMO3 (SMT3A, SMT3H1, SUMO-3, Smt3B)
Fusion protein or tag:
EmGFP
Terminal:
N terminal on backbone
Vector backbone:
pcDNA6.2-GW/EmGFP
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression, RNAi
Backbone size w/o insert (bp):
5699
Cloning site 5':
attB1
Site destroyed during cloning:
Unknown
Cloning site 3':
attB2
Site destroyed during cloning:
Unknown
5' sequencing primer:
EGFP-C List of Sequencing Primers
Bacterial resistance(s)
Spectinomycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Wulf Paschen
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Gene expression and cell growth are modified by silencing SUMO2 and SUMO3 expression. Yang et al (Biochem Biophys Res Commun. 2009 Apr 24. 382(1):215-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31073" in your Materials and Methods section.