Addgene - pIRESneo-mTOR-RR/KI Plasmid Data

Plasmid 31074: pIRESneo-mTOR-RR/KI

Gene/insert name:
mTOR
Insert size:
7600
Species:
H. sapiens (human)
GenBank ID:
NM_004958 NM_002475
Entrez Gene:
MTOR (FRAP, FRAP1, FRAP2, RAFT1, RAPT1)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Mutation:
S2035T D2357E
Vector backbone:
pIRESneo
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5300
Cloning site 5':
NotI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (3)
Principal Investigator:
Jie Chen
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Mammalian target of rapamycin (mTOR) signaling is required for a late-stage fusion process during skeletal myotube maturation. Park et al (J Biol Chem. 2005 Sep 9. 280(36):32009-17. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31074" in your Materials and Methods section.