Addgene - pSR-puro-Sec5 Plasmid Data

Plasmid 31117: pSR-puro-Sec5

Gene/insert name:
Sec5 shRNA
Alt name:
SEC5
shRNA sequence:
GGTCGGAAAGACAAGGCAGAT
Species:
H. sapiens (human)
Entrez Gene:
EXOC2 (RP11-164H16.2, FLJ11026, SEC5, SEC5L1, Sec5p)
Vector backbone:
pSUPER.retro.puro
(Search Vector Database)
Backbone manufacturer:
Oligoengine
Vector type:
Mammalian Expression, Retroviral, RNAi
Backbone size w/o insert (bp):
6349
5' sequencing primer:
pLXSN 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Christopher Counter
Terms and Licenses:
MTA

Comments:

shRNA Sec5 sequence:
5'-GGTCGGAAAGACAAGGCAGAT-3'

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Sec5 and Exo84 foster oncogenic ras-mediated tumorigenesis. Issaq et al (Mol Cancer Res. 2010 Feb . 8(2):223-31. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31117" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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