pCMV-Tag4A-MITF-M (E87R)
(Plasmid #31152)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCMV-TAG4A
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    MITF-M
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1257
  • Mutation
    Glutamic acid 87 changed to Arginine
  • GenBank ID
    NM_198158
  • Entrez Gene
    MITF (a.k.a. CMM8, MI, WS2, WS2A, bHLHe32)
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Tag4A-MITF-M (E87R) was a gift from Yardena Samuels (Addgene plasmid # 31152)