Addgene - 474 p5E-basprom Plasmid Data

Plasmid 31158: 474 p5E-basprom

Gene/insert name:
basal adenovirus E1b TATA element
Alt name:
transcriptional start site from carp b-actin gene
Vector backbone:
p5E
(Search Vector Database)
Vector type:
Entry vector
Backbone size w/o insert (bp):
2810
Cloning method:
Gateway Cloning
Cloning site 5':
Gateway
Site destroyed during cloning:
No
Cloning site 3':
Gateway
Site destroyed during cloning:
No
5' sequencing primer:
M13pUC-fwd List of Sequencing Primers
3' sequencing primer:
M13rev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Nathan Lawson
Terms and Licenses:
MTA

Comments:

"p5E" refers to 5' entry clones flanked by L4-R1

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31158" in your Materials and Methods section.