Addgene - pDEST-HemmarG Plasmid Data

Plasmid 31221: pDEST-HemmarG

Gene/insert name:
CD4-tdGFP
Insert size:
2340
Species:
H. sapiens (human), D. melanogaster (fly)
Species:
Aequorea victoria
Entrez Gene:
CD4 (CD4mut)
Fusion protein or tag:
CD4
Terminal:
N terminal on insert
Fusion protein or tag:
EGFP/GFP
Terminal:
C terminal on insert
Mutation:
Fusion of signal peptide of Drosophila Akh gene, human CD4 transmembrane domain, EGFP, GFP, and Kir2.1 ER exit signal.
Vector backbone:
pAPIC-HIH
(Search Vector Database)
Vector type:
Insect Expression
Backbone size w/o insert (bp):
10193
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
ACTGCAACTACTGAAATCTGCC List of Sequencing Primers
3' sequencing primer:
GGCGCACAGAAATGATTACAAC
Bacterial resistance(s)
Ampicillin
Growth strain(s)
ccdB Survival
Growth temperature (℃):
37
Growth instructions:
ccdB Survival™ 2 T1R Cells @ 37C.
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Yuh-Nung Jan
Terms and Licenses:
MTA

Comments:

Gateway destination vector for cloning any enhancer to drive expression of membrane marker CD4-tdGFP

The depositing laboratory recommends growing bacteria either on LB plates with 80 ug/ml Carbenicillin or in LB liquid media with 60 ug/ml Carbenicillin (a semi-synthetic ampicillin analog)

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Enhancer-driven membrane markers for analysis of nonautonomous mechanisms reveal neuron-glia interactions in Drosophila. Han et al (Proc Natl Acad Sci U S A. 2011 May 23. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31221" in your Materials and Methods section.