Addgene - pKEB31 Plasmid Data

Plasmid 31224: pKEB31

Gene/insert name:
ccdB and Cm resistance gene
Insert size:
1447
Vector backbone:
pDD62
(Search Vector Database)
Backbone size w/o insert (bp):
12617
Cloning method:
Gateway Cloning
5' sequencing primer:
CAT-R (for 1st attR) List of Sequencing Primers
3' sequencing primer:
Tet-R (for 2nd attR)
Bacterial resistance(s)
Chloramphenicol
Growth strain(s)
Unknown
Growth temperature (℃):
37
Growth instructions:
ccdB survival
High or low copy:
Unknown
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Adam Bogdanove
Principal Investigator:
Daniel Voytas
Terms and Licenses:
MTA
Cellectis Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak et al (Nucleic Acids Res. 2011 Apr 14. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31224" in your Materials and Methods section.