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pfGAD65
(Plasmid #31231)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCRII-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3950
  • Vector type
    cDNA for in situ hybridization

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    GAD2
  • Alt name
    GAD65
  • Species
    Mustela putorius furo
  • Insert Size (bp)
    1015

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ cloning site TOPO (unknown if destroyed)
  • 3′ cloning site TOPO (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

For riboprobe cut with EcoRI. Runoffs with SP6.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pfGAD65 was a gift from Cliff Ragsdale (Addgene plasmid # 31231)
  • For your References section:

    Molecular analysis of neocortical layer structure in the ferret. Rowell JJ, Mallik AK, Dugas-Ford J, Ragsdale CW. J Comp Neurol. 2010 Aug 15. 518(16):3272-89. 10.1002/cne.22399 PubMed 20575059