Addgene - pfGAD65 Plasmid Data

Plasmid 31231: pfGAD65

Gene/insert name:
GAD2
Alt name:
GAD65
Insert size:
1015
Species:
Mustela putorius furo
Vector backbone:
pCRII-TOPO
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
cDNA for in situ hybridization
Backbone size w/o insert (bp):
3950
Cloning method:
TOPO Cloning
Cloning site 5':
TOPO
Site destroyed during cloning:
Unknown
Cloning site 3':
TOPO
Site destroyed during cloning:
Unknown
5' sequencing primer:
SP6 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin and Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Cliff Ragsdale
Terms and Licenses:
MTA

Comments:

For riboprobe cut with EcoRI. Runoffs with SP6.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Molecular analysis of neocortical layer structure in the ferret. Rowell et al (J Comp Neurol. 2010 Aug 15. 518(16):3272-89. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31231" in your Materials and Methods section.