hPDI1-18-508_C400S_C56S-pFLAG-CMV1
(Plasmid #31383)

• Depositing Lab: David Ron
• Publication: Zito et al Mol Cell. 2010 Dec 10. 40(5):787-97.

Backbone

• Vector backbone: pFLAG-CMV1
• Backbone size w/o insert (bp): 4732
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PDI1A
• Alt name: prolyl 4-hydroxylase, beta polypeptide
• Species: H. sapiens (human)
• Insert Size (bp): 1514
• Mutation: C-terminal cysteines of both active sites mutated to serine (C400S and C56S)
• Entrez Gene: P4HB (a.k.a. CLCRP1, DSI, ERBA2L, GIT, P4Hbeta, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB)
• Tag / Fusion Protein:
  • FLAG M2 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV_for
• 3′ sequencing primer: CMVR

Resource Information

• A portion of this plasmid was derived from a plasmid made by: A bacterial expression vector for human PDI1 (hPDI1(18-508)pTrcHis-A) was a gift of Professor Colin Thorpe of U Delaware

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry