|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31390||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerStephen Elledge
- Backbone size w/o insert (bp) 1955
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Growth instructionsBW23474 (pir::116), LB 37°C
Copy numberLow Copy
Alt nameaminoglycoside phosphotransferase
Insert Size (bp)1474
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (destroyed during cloning)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer n/a (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byPromega, Madison, WI, USA
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pINS-Neo was a gift from Yegor Vassetzky (Addgene plasmid # 31390)
For your References section:A set of vectors for introduction of antibiotic resistance genes by in vitro Cre-mediated recombination. Dmitriev PV, Vassetzky YS. BMC Res Notes. 2008 . 1():135. 10.1186/1756-0500-1-135 PubMed 19105832
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.