pINS-Neo
(Plasmid #31390)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pUNI10
  • Backbone manufacturer
    Stephen Elledge
  • Backbone size w/o insert (bp) 1955
  • Vector type
    Cre/Lox
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BW23474
  • Growth instructions
    BW23474 (pir::116), LB 37°C
  • Copy number
    Low Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    aph
  • Alt name
    aminoglycoside phosphotransferase
  • Species
    Tn5
  • Insert Size (bp)
    1474

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Promega, Madison, WI, USA
  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pINS-Neo was a gift from Yegor Vassetzky (Addgene plasmid # 31390)
  • For your References section:

    A set of vectors for introduction of antibiotic resistance genes by in vitro Cre-mediated recombination. Dmitriev PV, Vassetzky YS. BMC Res Notes. 2008 . 1():135. 10.1186/1756-0500-1-135 PubMed 19105832