pEG3a
(Plasmid
#31398)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31398 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBAD33
- Backbone size w/o insert (bp) 5352
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsAny E. coli strain
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namezapB
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Alt nameyiiU
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SpeciesE. coli
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Insert Size (bp)973
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GenBank IDECK3920
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Entrez GenezapB (a.k.a. ECK3920, JW3899, yiiU)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (destroyed during cloning)
- 3′ cloning site SmaI (destroyed during cloning)
- 5′ sequencing primer Not commercial
- 3′ sequencing primer Not commercial (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEG3a was a gift from Kenn Gerdes (Addgene plasmid # 31398 ; http://n2t.net/addgene:31398 ; RRID:Addgene_31398) -
For your References section:
Novel coiled-coil cell division factor ZapB stimulates Z ring assembly and cell division. Ebersbach G, Galli E, Moller-Jensen J, Loewe J, Gerdes K. Mol Microbiol. 2008 May . 68(3):720-35. 10.1111/j.1365-2958.2008.06190.x PubMed 18394147