|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31450||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6380
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHNF4A 3'UTR fragments
SpeciesH. sapiens (human)
Insert Size (bp)358
Mutationmut2 in balancer
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer taacaccgagttcgtgaaggtg (Common Sequencing Primers)
Terms and Licenses
To measure regulatory potential of 3'UTR: balancer with mut2 and negative element A
Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRLCon183-221mut2_850-1207 was a gift from Gerhart Ryffel (Addgene plasmid # 31450 ; http://n2t.net/addgene:31450 ; RRID:Addgene_31450)
For your References section:A systematic analysis of the 3'UTR of HNF4A mRNA reveals an interplay of regulatory elements including miRNA target sites. Wirsing A, Senkel S, Klein-Hitpass L, Ryffel GU. PLoS One. 2011;6(11):e27438. Epub 2011 Nov 30. 10.1371/journal.pone.0027438 PubMed 22140441