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(Plasmid #31463)

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Available to Academic and Nonprofits Only


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2850
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Grow TopTen cells at 30C instead of 37C to reduce risk of recombination at the SV40 pA sites; the backbone is flipped relative to parental Clontech pTRETightBI plasmid.
  • Copy number

Sequence Information


  • Gene/Insert name
    eYFP and mCherry
  • Species
    fluorescent proteins
  • Insert Size (bp)
  • Tag / Fusion Protein
    • NLS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI, BamHI (not destroyed)
  • 3′ cloning site NdeI, ClaI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

0 tandem miR-20 binding sites in mCherry 3'UTR

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRETightBI-RY-0 was a gift from Phil Sharp (Addgene plasmid # 31463)
  • For your References section:

    MicroRNAs can generate thresholds in target gene expression. Mukherji S, Ebert MS, Zheng GX, Tsang JS, Sharp PA, van Oudenaarden A. Nat Genet. 2011 Aug 21;43(9):854-9. doi: 10.1038/ng.905. 10.1038/ng.905 PubMed 21857679