pKin1C
(Plasmid #31605)

• Depositing Lab: Anthony Brown
• Publication: Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-5006.

Backbone

• Vector backbone: pEGFP-C1 (EGFP removed)
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4001
• Modifications to backbone: Deleted EGFP (601-1390) from original pEGFP-C1 vector
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: E. coli DH5α, 16hrs at 37C
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: kinesin-1C
• Alt name: KIF5C
• Species: M. musculus (mouse)
• Insert Size (bp): 3890
• GenBank ID: BC067051
• Entrez Gene: Kif5c (a.k.a. KINN, Khc, NKHC, NKHC2)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: specific sequence primer
• 3′ sequencing primer: specific sequence primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Kinesin-1C cDNA (GenBank accession BC067051, I.M.A.G.E. clone:30536079, MGC:86067) was obtained from Open Biosystems (Huntsville, AL)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry