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Plasmid
31618:
pCS2-YFP-Smo
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Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result. Lateral transport of Smoothened from the plasma membrane to the membrane of the cilium. Milenkovic et al (J Cell Biol. 2009 Nov 2. 187(3):365-74. PubMed) Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31618" in your Materials and Methods section. |
Wild-type mouse Smo was tagged at the N terminus (with the insertion C terminal to the signal sequence) with YFP.