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Plasmid 31618: pCS2-YFP-Smo
  • Smo

  • M. musculus (mouse)

  • Smo (E130215L21Rik, Smoh, bnb, smoothened)

  • YFP

  • N terminal on insert

  • pCS2+
    (Search Vector Database)

  • RZPD

  • Mammalian Expression, Worm Expression ; Zebrafish, Avian Expression

  • 4095

  • EGFP-C List of Sequencing Primers

  • M13_puc_Rev

  • Ampicillin

  • DH5alpha

  • 37

  • Low Copy

  • View sequences (1)
  • Rajat Rohatgi

  • MTA

Comments: 

Wild-type mouse Smo was tagged at the N terminus (with the insertion C terminal to the signal sequence) with YFP.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Lateral transport of Smoothened from the plasma membrane to the membrane of the cilium. Milenkovic et al (J Cell Biol. 2009 Nov 2. 187(3):365-74. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31618" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only