pGEX-BAIAP2 wt
(Plasmid #31675)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    BAI1-associated protein 2
  • Alt name
    GST-BAIAP2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1550
  • Entrez Gene
    BAIAP2 (a.k.a. BAP2, FLAF3, IRSP53)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer pGEX 5'
  • 3′ sequencing primer pGEX 3'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Open Biosystems Human Orfeome Collection
  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-BAIAP2 wt was a gift from Anne Brunet (Addgene plasmid # 31675)
  • For your References section:

    Chemical Genetic Screen for AMPKalpha2 Substrates Uncovers a Network of Proteins Involved in Mitosis. Banko MR, Allen JJ, Schaffer BE, Wilker EW, Tsou P, White JL, Villen J, Wang B, Kim SR, Sakamoto K, Gygi SP, Cantley LC, Yaffe MB, Shokat KM, Brunet A. Mol Cell. 2011 Dec 23;44(6):878-92. Epub 2011 Dec 1. 10.1016/j.molcel.2011.11.005 PubMed 22137581