pDONR223-MCU
(Plasmid #31726)

• Depositing Lab: Vamsi Mootha
• Publication: Baughman et al Nature. 2011 Jun 19. ():.

Backbone

• Vector backbone: pDONR223
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2787
• Modifications to backbone: None
• Vector type: pDONR Gateway

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10/P3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MCU
• Alt name: CCDC109A
• Species: H. sapiens (human)
• Insert Size (bp): 1056
• Mutation: *
• GenBank ID: NM_138357
• Entrez Gene: MCU (a.k.a. C10orf42, CCDC109A, HsMCU)
• Promoter: None
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 (-21) Forward
• 3′ sequencing primer: M13 Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Marc Vidal's lab originally made this construct (PMID15489335). Via collaboration we received it and sequence verified it for use in the paper associated with this entry. The Vidal/Mootha collaboration was cited in Pagliarini et al, 2008, Cell. (PMID 18614015)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*A3T aa residue substitution in MCU, which is not known to affect protein function