pBMN YFP-LID
(Plasmid #31767)

• Depositing Lab: Thomas Wandless
• Publication: Bonger et al Nat Chem Biol. 2011 Jul 3. doi: 10.1038/nchembio.598.

Backbone

• Vector backbone: pBMN
• Backbone manufacturer: Garry Nolan
• Backbone size w/o insert (bp): 6943
• Vector type: Retroviral
• Selectable markers: HcRed fluorescence behind IRES

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Top10/P3
• Growth instructions: TOP10 cells. Culture at 30C to prevent recombination of LTR and lost insert. Takes >20 hours to get good culture at 30C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: YFP-LID
• Alt name: YFP fused to FKBP12 derived LID domain
• Species: H. sapiens (human)
• Insert Size (bp): 1156
• Mutation: F36V on FKBP12 and additional 19 aa (TRGVEEVAEGVVLLRRRGN) on C terminus of FKBP12
• GenBank ID: NM_000801
• Entrez Gene: FKBP1A (a.k.a. FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE)
• Promoter: LTR
• Tags / Fusion Proteins:
  • YFP (N terminal on insert)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NsiI (not destroyed)
• 5′ sequencing primer: pBMN vector specific

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry