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pBMN YFP-LID
(Plasmid #31767)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31767 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBMN
  • Backbone manufacturer
    Garry Nolan
  • Backbone size w/o insert (bp) 6943
  • Vector type
    Retroviral
  • Selectable markers
    HcRed fluorescence behind IRES

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Top10/P3
  • Growth instructions
    TOP10 cells. Culture at 30C to prevent recombination of LTR and lost insert. Takes >20 hours to get good culture at 30C
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    YFP-LID
  • Alt name
    YFP fused to FKBP12 derived LID domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1156
  • Mutation
    F36V on FKBP12 and additional 19 aa (TRGVEEVAEGVVLLRRRGN) on C terminus of FKBP12
  • GenBank ID
    NM_000801
  • Promoter LTR
  • Tags / Fusion Proteins
    • YFP (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NsiI (not destroyed)
  • 5′ sequencing primer pBMN vector specific
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBMN YFP-LID was a gift from Thomas Wandless (Addgene plasmid # 31767)
  • For your References section:

    Small-molecule displacement of a cryptic degron causes conditional protein degradation. Bonger KM, Chen LC, Liu CW, Wandless TJ. Nat Chem Biol. 2011 Jul 3. doi: 10.1038/nchembio.598. 10.1038/nchembio.598 PubMed 21725303