Addgene - pCDF1-GRM3 (G561E) Plasmid Data

Plasmid 31799: pCDF1-GRM3 (G561E)

Gene/insert name:
GRM3
Alt name:
mGluR3
Insert size:
2640
Species:
H. sapiens (human)
GenBank ID:
NM_000840
Entrez Gene:
GRM3 (GLUR3, GPRC1C, MGLUR3, mGlu3)
Fusion protein or tag:
FLAG
Terminal:
C terminal on insert
Mutation:
Changed glycine 561 to glutamic acid
Vector backbone:
pCDF1- MCS2-EF1-puromycin
(Search Vector Database)
Backbone manufacturer:
SBI
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
6600
Promoter:
CMV
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
NA List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH10B
Growth temperature (℃):
37
High or low copy:
Low Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Principal Investigator:
Yardena Samuels
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31799" in your Materials and Methods section.