pCDF1-GRM3 (E870K)
(Plasmid
#31802)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31802 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCDF1- MCS2-EF1-puromycin
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Backbone manufacturerSBI
- Backbone size w/o insert (bp) 6600
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGRM3
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Alt namemGluR3
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SpeciesH. sapiens (human)
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Insert Size (bp)2640
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MutationChanged glutamic acid 870 to lysine
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GenBank IDNM_000840
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Entrez GeneGRM3 (a.k.a. GLUR3, GPRC1C, MGLUR3, mGlu3)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDF1-GRM3 (E870K) was a gift from Yardena Samuels (Addgene plasmid # 31802 ; http://n2t.net/addgene:31802 ; RRID:Addgene_31802) -
For your References section:
Exon capture analysis of G protein-coupled receptors identifies activating mutations in GRM3 in melanoma. Prickett TD, Wei X, Cardenas-Navia I, Teer JK, Lin JC, Walia V, Gartner J, Jiang J, Cherukuri PF, Molinolo A, Davies MA, Gershenwald JE, Stemke-Hale K, Rosenberg SA, Margulies EH, Samuels Y. Nat Genet. 2011 Sep 25;43(11):1119-26. doi: 10.1038/ng.950. 10.1038/ng.950 PubMed 21946352