Addgene - pSC300 Plasmid Data

Plasmid 31820: pSC300

Gene/insert name:
GFP (lacking ATG start codon)
Insert size:
723
Vector backbone:
pSODIT
(Search Vector Database)
Backbone manufacturer:
Koen De Smet Imperial College Medical School
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5497
Promoter:
SOD
Promoter:
SOD
Cloning site 5':
HindIII
Site destroyed during cloning:
Yes
Cloning site 3':
HindIII
Site destroyed during cloning:
Yes
5' sequencing primer:
NA List of Sequencing Primers
3' sequencing primer:
GFP-R
Bacterial resistance(s)
Hygromycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Yossef Av-Gay
Terms and Licenses:
MTA

Comments:

Plasmid can also be grown in mycobacteria.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Monitoring promoter activity and protein localization in Mycobacterium spp. using green fluorescent protein. Cowley et al (Gene. 2001 Feb 21;264(2):225-31. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31820" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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