pcDNA3-YFP-PKD2
(Plasmid #31821)

• Depositing Lab: Alexandra Newton
• Publication: Kunkel et al J Biol Chem. 2009 Sep 4. 284(36):24653-61.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YFP-Protein Kinase D2
• Alt name: YFP-PKD2
• Alt name: PKD2
• Species: H. sapiens (human)
• Insert Size (bp): 2655
• GenBank ID: AF309082.1
• Entrez Gene: PRKD2 (a.k.a. HSPC187, PKD2, nPKC-D2)
• Tag / Fusion Protein:
  • YFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry