Addgene - pSicoR-Ef1a-mCh-GFPi Plasmid Data

Plasmid 31849: pSicoR-Ef1a-mCh-GFPi

Gene/insert name:
GFP RNAi
shRNA sequence:
ACAGCCACAACGTCTATAT
Vector backbone:
pSicoR-Ef1a-mCh
(Search Vector Database)
Backbone manufacturer:
Addgene plasmid #31847
Vector type:
Mammalian Expression, Lentiviral, RNAi, Cre/Lox
Backbone size w/o insert (bp):
7525
Promoter:
U6 for shRNA; Ef1alpha for mCherry
Cloning site 5':
HpaI
Site destroyed during cloning:
Yes
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
U6 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Supplemental document:
pSicoR-Ef1a-mCh-GFPi_Seq (application/pdf)
Supplemental document:
pSicoR-Ef1a-mCh-GFPi_txt (text/plain)
Supplemental document:
pSicoR-Ef1a-mCh-GFPi.gb (application/octet-stream)
Principal Investigator:
Bruce Conklin
Terms and Licenses:
MTA
Clontech Limited Use Label License

Comments:

The pSicoR-Ef1α-mCh-Puro lentiviral construct was created by replacing the CMV promoter of pSicoR-mCherry (Addgene vector 21907) with Ef1α.

This vector can be used as an shRNA control.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Alternative splicing regulates mouse embryonic stem cell pluripotency and differentiation. Salomonis et al (Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10514-9. Epub 2010 May 24. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31849" in your Materials and Methods section.