pShuttle-CMV-iRFP
(Plasmid #31856)

• Purpose: a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging
• Depositing Lab: Vladislav Verkhusha
• Publication: Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.1038/nbt.1918.

Backbone

• Vector backbone: pShuttle-CMV
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 7384
• Vector type: Mammalian Expression, Adenoviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: iRFP
• Alt name: iRFP713
• Species: Rhodopseudomonas palustris
• Insert Size (bp): 948
• Mutation: T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T and N290Y relative to RpBphP2
• GenBank ID: JN247409
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • Verkhusha lab feature article
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For additional protocols on using iRFP in other tissue types please review:
Verkhusha. Deep-tissue photoacoustic tomography of a genetically encoded near-infrared fluorescent probe. Angewandte Chemie Int. Ed. 2012, 51: 1448-1451.
PubMed: https://www.ncbi.nlm.nih.gov/pubmed/22213541/

For more information on in vivo imaging, please see:
https://www.addgene.org/fluorescent_proteins/in_vivo/