Addgene - pCR4-24XMS2SL-stable Plasmid Data

Plasmid 31865: pCR4-24XMS2SL-stable

Gene/insert name:
24xMS2 Stem Loops - Stable
Insert size:
1300
Vector backbone:
pCR4Blunt-TOPO
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Backbone size w/o insert (bp):
3956
Cloning method:
TOPO Cloning
5' sequencing primer:
M13F List of Sequencing Primers
3' sequencing primer:
M13R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Robert Singer
Terms and Licenses:
MTA

Comments:

This version of the 24xMS2 stem loops has been engineered to allow stable propagation in bacteria. Loss of repeats is still possible, but occurs significantly less often than with previous version. This plasmid is considered the replacement for pSL-MS2-24X (Addgene plasmid# 27120).

The 24xMS2 stem loop cassettes in this plasmid were generated from two non-identical stem-loops to reduce unnecessary redundancy and the chance of recombination in bacteria. The sequence for a single cassette (with the two non-identical stem-loops in uppercase) is: tacggtacttattgccaagaaaGCACGAGCATCAGCCGTGC ctccaggtcgaatcttcaaaCGACGACGATCACGCGTCGctccagtattccagggttcatc

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Localization of ASH1 mRNA particles in living yeast. Bertrand et al (Mol Cell. 1998 Oct . 2(4):437-45. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31865" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

31863: pET22HT-PP7delFG