Addgene - pTight-9-124 Plasmid Data

Plasmid 31874: pTight-9-124

Gene/insert name:
miR-9/9* and miR-124
Alt name:
miR-9/9* and miR-124 pri-transcripts
Insert size:
655
Species:
M. musculus (mouse)
GenBank ID:
NR_029818 NR_029814
Vector backbone:
pTLemiR
(Search Vector Database)
Backbone manufacturer:
home made/Crabtree lab
Vector type:
Lentiviral
Cloning method:
Ligation Independent Cloning
5' sequencing primer:
CACTTCGTGGACCACAGACTGG List of Sequencing Primers
3' sequencing primer:
GCACACCGGCCTTATTCCAAG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
30
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Jerry Crabtree
Terms and Licenses:
MTA

Comments:

Addgene's quality control sequence is missing the following sequence upstream of IRES - "CCGCAAATT". This deletion should not affect plasmid function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: MicroRNA-mediated conversion of human fibroblasts to neurons. Yoo et al (Nature. 2011 Jul 13. doi: 10.1038/nature10323. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31874" in your Materials and Methods section.