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(Plasmid #31874)


Item Catalog # Description Quantity Price (USD)
Plasmid 31874 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    home made/Crabtree lab
  • Vector type
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    miR-9/9* and miR-124
  • Alt name
    miR-9/9* and miR-124 pri-transcripts
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • GenBank ID
    NR_029818 NR_029814

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CACTTCGTGGACCACAGACTGG
  • 3′ sequencing primer GCACACCGGCCTTATTCCAAG
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Addgene's quality control sequence is missing the following sequence upstream of IRES - "CCGCAAATT". This deletion should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTight-9-124 was a gift from Jerry Crabtree (Addgene plasmid # 31874)
  • For your References section:

    MicroRNA-mediated conversion of human fibroblasts to neurons. Yoo AS, Sun AX, Li L, Shcheglovitov A, Portmann T, Li Y, Lee-Messer C, Dolmetsch RE, Tsien RW, Crabtree GR. Nature. 2011 Jul 13. doi: 10.1038/nature10323. 10.1038/nature10323 PubMed 21753754