Addgene - pLKO-puro FLAG wildtype, catalytic active lipin 1 Plasmid Data

Plasmid 32010: pLKO-puro FLAG wildtype, catalytic active lipin 1

Gene/insert name:
lipin1
Alt name:
fld; Lpin1; Kiaa0188; mKIAA0188; 4631420P06
Species:
M. musculus (mouse)
GenBank ID:
NM_001130412
Entrez Gene:
Lpin1 (4631420P06, Kiaa0188, Lipin1, fld, mKIAA0188)
Fusion protein or tag:
FLAG
Terminal:
N terminal on backbone
Vector backbone:
pLKO.1-puro
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
7100
Modifications to Backbone:
U6 replaced by CMV and FLAG added N-term to insert. 5->3': CMV-> FLAG -> sal I restriction site -> 1 base(to keep in frame) -> cDNA -> Not I restriction site
Promoter:
CMV
Cloning site 5':
SalI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
CMV forward List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
David Sabatini
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Peterson et al (Cell. 2011 Aug 5;146(3):408-20. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32010" in your Materials and Methods section.