Gene/insert name: PIWIL2
Alt name: PDB: 3QIR
Alt name: piwi-like 2, PAZ domain
Insert size: 444
Species:
H. sapiens (human)
GenBank ID:
BC111751
Entrez Gene:
PIWIL2
(CT80, FLJ10351, HILI, MGC133049, PIWIL1L, mili)
Fusion protein or tag: His (with TEV cleavage)
Terminal: N terminal on backbone
Mutation: PAZ domain only (amino acids 386-533)
Vector backbone:
pET28-MHLSearch Vector Database )
Backbone manufacturer:
Structural Genomics Consortium (Addgene plasmid 26096)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
7325
Cloning method: Ligation Independent Cloning
5' sequencing primer:
T7
List of Sequencing Primers
3' sequencing primer:
T7-term
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence:
View sequences (2)
Principal Investigator:
Cheryl Arrowsmith
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter
Addgene has sequenced a portion of this plasmid for verification.
Click here for the sequencing
result.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
32042" in your Materials and Methods section.
PDB: 3QIR. http://www.thesgc.org/structures/details?pdbid=...