Addgene - SMYD3 Plasmid Data

Plasmid 32048: SMYD3

Gene/insert name:
SMYD3
Alt name:
PDB: 3QWP
Alt name:
SET and MYND domain containing 3, methyltransferase domain
Insert size:
1284
Species:
H. sapiens (human)
Entrez Gene:
SMYD3 (FLJ21080, KMT3E, MGC104324, ZMYND1, ZNFN3A1, bA74P14.1)
Fusion protein or tag:
His (with TEV cleavage)
Terminal:
N terminal on backbone
Vector backbone:
pET28-MHL
(Search Vector Database)
Backbone manufacturer:
Structural Genomics Consortium (Addgene plasmid 26096)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
7325
Cloning method:
Ligation Independent Cloning
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T7-term
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Cheryl Arrowsmith
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Comments:

PDB: 3QWP. http://www.thesgc.org/structures/details?pdbid=...

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32048" in your Materials and Methods section.