Addgene - pBabe EGFR Del5(T790M) Plasmid Data

Plasmid 32069: pBabe EGFR Del5(T790M)

Gene/insert name:
EGFR
Insert size:
3600
Species:
H. sapiens (human)
Entrez Gene:
EGFR (ERBB, ERBB1, HER1, PIG61, mENA)
Mutation:
Delete E746-S752, insert V, T790M
Vector backbone:
pBABEpuro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5169
Cloning site 5':
SnaB1
Site destroyed during cloning:
Yes
Cloning site 3':
SnaB1
Site destroyed during cloning:
Yes
5' sequencing primer:
pBABE 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Principal Investigator:
Matthew Meyerson
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Allele-dependent variation in the relative cellular potency of distinct EGFR inhibitors. Yuza et al (Cancer Biol Ther. 2007 May . 6(5):661-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32069" in your Materials and Methods section.