Addgene - pAd GFP HDAC5 WT Plasmid Data

Plasmid 32217: pAd GFP HDAC5 WT

Gene/insert name:
HDAC5
Insert size:
3400
Species:
H. sapiens (human)
GenBank ID:
NM_001015053
Entrez Gene:
HDAC5 (HD5, NY-CO-9)
Fusion protein or tag:
GFP
Terminal:
C terminal on insert
Vector backbone:
pAd CMV/V5 DEST
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression, Adenoviral
Backbone size w/o insert (bp):
37000
Cloning method:
Gateway Cloning
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
GFP-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
Subcloned and converted into a Gateway plasmid from original plasmid described previously in Berdeux et al. PMID 17468767
Sequence: View sequences (2)
Principal Investigator:
Reuben Shaw
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Class IIa histone deacetylases are hormone-activated regulators of FOXO and mammalian glucose homeostasis. Mihaylova et al (Cell. 2011 May 13;145(4):607-21. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32217" in your Materials and Methods section.