LHP1468 - VPS23
(Plasmid #32239)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pRS316
  • Backbone manufacturer
    ATCC
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    VPS23
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    STP22 (a.k.a. YCL008C, VPL15, VPS23)
  • Promoter T7
  • Tag / Fusion Protein
    • HA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

HA tag is likely 3xHA and likely C-terminal

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LHP1468 - VPS23 was a gift from Linda Hicke (Addgene plasmid # 32239)