Addgene - TAL031 Plasmid Data

Plasmid 32271: TAL031

Gene/insert name:
TAL031
Alt name:
(Type III C-terminal- A/NI)
Alt name:
γ' / SNI / A
Insert size:
119
Vector backbone:
pUC57-ΔBsaI
(Search Vector Database)
Backbone size w/o insert (bp):
2708
Modifications to Backbone:
Silent valine mutation (C->G) at position 1794 to destroy alternate BsaI restriction site in pUC57
Cloning site 5':
BbsI
Site destroyed during cloning:
Yes
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
OK163 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Keith Joung
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sander et al (Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32271" in your Materials and Methods section.