Addgene - pMR150 Plasmid Data

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Plasmid 32292: pMR150

Gene/insert name:
genomic methylation probe-minor satellite repeat
Insert size:
150
Species:
M. musculus (mouse)
Vector backbone:
pBluescript II KS
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert:
2961
Cloning site 5':
PstI
Site destroyed during cloning:
Unknown
Cloning site 3':
PstI
Site destroyed during cloning:
Unknown
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Verne Chapman
Principal Investigator:
Rudolf Jaenisch
Terms and Licenses:
MTA

Comments:

Induction of tumors in mice by genomic hypomethylation. Gaudet F, Hodgson JG, Eden A, Jackson-Grusby L, Dausman J, Gray JW, Leonhardt H, Jaenisch R. Science. 2003 Apr 18;300(5618):489-92.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Differences in DNA methylation during oogenesis and spermatogenesis and their persistence during early embryogenesis in the mouse. Sanford et al (Genes Dev. 1987 Dec;1(10):1039-46. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32292" in your Materials and Methods section.