Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at Learn more

pCDNA3 5'cMyc-SMCl- S957A/S966A
(Plasmid #32372)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 32372 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
    pCDNA3 cMyc
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
  • GenBank ID
  • Entrez Gene
    SMC1A (a.k.a. CDLS2, DXS423E, SB1.8, SMC1, SMC1L1, SMC1alpha, SMCB)
  • Tag / Fusion Protein
    • c-Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ECORl (not destroyed)
  • 3′ cloning site Xhol (not destroyed)
  • 5′ sequencing primer CMV-f
  • 3′ sequencing primer BGHrev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3 5'cMyc-SMCl- S957A/S966A was a gift from Michael Kastan (Addgene plasmid # 32372)
  • For your References section:

    Involvement of the cohesin protein, Smc1, in Atm-dependent and independent responses to DNA damage. Kim ST, Xu B, Kastan MB. Genes Dev. 2002 Mar 1;16(5):560-70. 10.1101/gad.970602 PubMed 11877376