Addgene - pCDNA3 5'cMyc-SMCl- S957A/S966A Plasmid Data

Plasmid 32372: pCDNA3 5'cMyc-SMCl- S957A/S966A

Gene/insert name:
SMC1A
Alt name:
SMCB
Alt name:
CDLS2
Alt name:
SMCILI
Insert size:
3700
Species:
H. sapiens (human)
GenBank ID:
8243
Entrez Gene:
SMC1A (RP6-29D12.1, CDLS2, DXS423E, SB1.8, SMC1, SMC1L1, SMC1alpha, SMCB)
Fusion protein or tag:
c-Myc
Terminal:
N terminal on backbone
Mutation:
S957A/S996A
Vector backbone:
pCDNA3 cMyc
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
ECORl
Site destroyed during cloning:
No
Cloning site 3':
Xhol
Site destroyed during cloning:
No
5' sequencing primer:
CMV-f List of Sequencing Primers
3' sequencing primer:
BGHrev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Michael Kastan
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Involvement of the cohesin protein, Smc1, in Atm-dependent and independent responses to DNA damage. Kim et al (Genes Dev. 2002 Mar 1;16(5):560-70. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32372" in your Materials and Methods section.